Automated Sample Mounting and Alignment System for Biological Crystallography at a Synchrotron Source
نویسندگان
چکیده
منابع مشابه
Automation of sample mounting for macromolecular crystallography.
A standard sample holder and vial for cryocooled macromolecular crystals has been defined for use with robotic sample changers. This SPINE standard sample holder is a modified version, with added features and specifications, of sample holders in common use. In particular, the SPINE standard meets the precision required for automatic sample exchange and includes a cap that is identified by a two...
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Synchrotron radiation (SR) techniques are continuously pushing the frontiers of wavelength range usage, smaller crystal sample size, larger protein molecular weight and complexity, as well as better diffraction resolution. The new research specialism of probing functional states directly in crystals, via time-resolved Laue and freeze trapping structural studies, has been developed, with a range...
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The European Synchrotron Radiation Facility (ESRF) at Grenoble, France, is a 6 GeV machine producing hard X-radiation that can be used for pure and applied research in a wide range of disciplines including physics, chemistry, structural biology, materials science, the earth sciences, engineering and medicine. The overall nature of the machine will be described, including the features that give ...
متن کاملStrategies for macromolecular synchrotron crystallography.
Introduction During the past ten years the rate of protein structure determination by X-ray crystallography has risen about tenfold [1]. The use of synchrotron radiation was instrumental for this growth and the fraction of the results obtained this way increased from 28% in 1990 to 80% in 1999 (Figure 1). There are 52 synchrotron stations worldwide used for macromolecular crystallography, and t...
متن کاملISPyB: an information management system for synchrotron macromolecular crystallography
MOTIVATION Individual research groups now analyze thousands of samples per year at synchrotron macromolecular crystallography (MX) resources. The efficient management of experimental data is thus essential if the best possible experiments are to be performed and the best possible data used in downstream processes in structure determination pipelines. Information System for Protein crystallograp...
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ژورنال
عنوان ژورنال: Structure
سال: 2004
ISSN: 0969-2126
DOI: 10.1016/j.str.2004.03.011